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Robust Hot Start PCR with Convenient Room-Temperature Setup
- Hot Start Taq minimizes nonspecific amplification and primer-dimers
- Optimize reaction conditions with supplied Flexi Reaction Buffer and MgCl2 solution
- Direct-to-gel convenience
- Supplied at a concentration of 5u/µl
High-Performance Hot-Start PCR
GoTaq® Hot Start Polymerase contains high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94–95°C for two minutes. This enables hot-start PCR, where polymerase activity is eliminated or minimized at temperatures below 70°C. GoTaq® Hot Start Polymerase also exhibits 5´→3´ exonuclease activity. The enzyme is supplied with a tube of 25mM MgCl2 to optimize the magnesium concentration in your reactions.
GoTaq® Hot Start Polymerase with Colorless Flexi Buffer (C) and with Green Flexi Buffer (G) outperforms antibody (I) or chemically modified (A) competitor hot-start DNA polymerases for amplification of a 2.4kb fragment of the Human APC gene.
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GoTaq® Hot Start Polymerase Product Information