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Efficient Reverse Transcription of Templates with High Secondary Structure

• DNA polymerization using template DNA, RNA or RNA:DNA hybrids
• Requires a primer as well as Mg²⁺ or Mn²⁺
• Stability at higher reaction temperatures (37–58°C)

Reverse Transcriptase with Stability at Higher Temperatures

Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT) catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids. It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg²⁺ or Mn²⁺. The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.

Features

• *  High Concentration: Cat.# M9004 contains 600 units of AMV RT at 20–25u/μl.
• *  5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 250mM KCl, 50mM MgCl₂, 2.5mM spermidine, 50mM DTT.
• *  Temperature Stability: AMV RT is the preferred reverse transcriptase for templates with high secondary structure due to its stability at higher reaction temperatures (37–58°C).

Applications

• *  First- and second-strand synthesis of cDNA.
• *  Primer extensions and RNA sequencing.
• *  RT-PCR. 

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AMV Reverse Transcriptase Certificate of Analysis 9PIM510