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RNA-Dependent DNA Polymerase for cDNA Synthesis

• Enzyme supplied at a concentration of 200u/μl

RNA-Dependent DNA Polymerase for cDNA Synthesis

Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa. The RNase H activity of M-MLV RT is weaker than that of the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Advantages

• *  Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl₂, 50mM DTT.
• *  May Be Heat-Inactivated: M-MLV RT is inactivated by heating at 70°C for 10 minutes.

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M-MLV Reverse Transcriptase Certificate of Analysis 9PIM170