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Convenient Master Mixes for Probe-Based Real-Time RT-qPCR

• Ready-to-use mix of optimized components for robust  RT-qPCR using hydrolysis probes
• Compatible with fast and standard cycling methods on most real-time PCR instruments using TaqMan® and other probe assays such as molecular beacons
• Resistant to most PCR inhibitors

Reverse Transcription and qPCR in One Reaction

The GoTaq® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in a single-step real-time amplification reaction.

The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions. The GoTaq® Probe qPCR Master Mix includes dUTP. When dUTP is incorporated into the amplification products, the amplicons are susceptible to degradation by uracil-DNA glycosylase (UNG); allowing users to incorporate UNG into subsequent reactions for control of possible carryover contamination.

Expression levels for the Zmm19 gene in corn. Expression was evaluated via 1-step RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120).

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GoTaq® Probe 1-Step RT-qPCR System Technical Manual, TM379

GoTaq Probe 1-Step RT-qPCR Quick Protocol FB139