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홈 > 제품정보 > 시약 > 국내 공식 대리점 > GoTaq® Probe 2-Step RT-qPCR System, 2ml
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GoTaq® Probe 2-Step RT-qPCR System, 2ml

제품코드 : A6110

제품정보 : Real-time PCR

  • Hydrolysis probe detection 형식의 quantitative PCR assays에 최적화된 제품입니다.
  • 제품내 포함 된 시약을 이용해 RNA의 발현 및 정량을 다음의 두 과정으로 진행합니다
  • 1. GoScript™ Reverse Transcription System으로 first-strand cDNA 합성
  • 2. 합성된 cDNA와 GoTaq® Probe qPCR Master Mix를 이용해 실시간 정량분석

제품문의

  • 전화번호
    2292-8870

제품상세

Convenient Master Mixes for Probe-Based Real-Time qPCR and RT-qPCR

  • Ready-to-use mix of optimized components for robust qPCR and RT-qPCR using hydrolysis probes
  • Compatible with fast and standard cycling methods on most real-time PCR instruments using TaqMan® and other probe assays such as molecular beacons
  • Resistant to most PCR inhibitors

Two-Step Reverse Transcription and qPCR

The GoTaq® Probe 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® Probe qPCR Master Mix.

The GoTaq® Probe qPCR Master Mix contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer. The master mix does not contain a reference dye; a separate tube of CXR reference dye is included.

The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.




Amplification curve for GAPDH detection from human pancreas RNA using the GoTaq® Probe 2-Step RT-qPCR System.

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