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Convenient Master Mixes for Probe-Based Real-Time qPCR and RT-qPCR

• Ready-to-use mix of optimized components for robust qPCR using hydrolysis probes
• Compatible with fast and standard cycling methods on most real-time PCR instruments using TaqMan® and other probe assays such as molecular beacons
• Resistant to most PCR inhibitors

Sensitivity and Specificity for qPCR

The GoTaq® Probe qPCR Master Mix is a ready-to-use, stabilized, 2X formulation with all of the components for quantitative PCR (qPCR). Just add template, primers and probe. Optimized for fast and reproducible quantitative PCR assays in the hydrolysis probe detection format, the master mix contains GoTaq® Hot Start Polymerase, MgCl₂, dNTPs and a proprietary reaction buffer. The master mix does not contain a reference dye; a separate tube of CXR reference dye is included.

The GoTaq® Probe qPCR Master Mix offers:

• *  Sensitive detection for earlier quantitation of low- and high-copy targets over a broad dynamic range.
• *  Optimized formulation compatible with running multiplex assays.
• *  Room-temperature setup, making it suitable for automation and high-throughput detection.
• *  Resistance to a wide range of PCR inhibitors.

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GoTaq Probe qPCR Master Mix TM378

GoTaq Probe qPCR Master Mix Quick Protocol FB138