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홈 > 제품정보 > 시약 > 국내 공식 대리점 > GoTaq® 1-Step RT-qPCR System, 5ml
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GoTaq® 1-Step RT-qPCR System, 5ml

제품코드 : A6020

제품정보 : Real-time PCR

  • Dye-based (BRYT Green® Dye) quantitative PCR assays 제품입니다.
  • 1-step RT-qPCR master mix: 제품 내에 GoScript™ Reverse Transcriptase 와 GoTaq® qPCR Master Mix가 포함되 있어 한번의 반응으로 RNA의 발현을 검출 및 정량할 수 있습니다.

제품문의

  • 전화번호
    2292-8870

제품상세

Convenient Master Mixes for Real-Time RT-qPCR

  • Ready-to-use mix of optimized components for dye-based RT-qPCR
  • Compatible with both fast and standard cycling methods
  • Resistant to a wide range of PCR inhibitors

Simple, Sensitive, Dye-Based qPCR and RT-qPCR

The GoTaq® RT-qPCR Systems are ready-to-use, 2X master mixes containing BRYT Green® Dye, a  fluorescent DNA binding dye with minimal PCR inhibition, providing maximum amplification efficiency and greater fluorescence enhancement than SYBR® Green I.


Reverse Transcription and qPCR in One Reaction

The GoTaq® 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step real-time RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix in a single reaction well. The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions.

Other features provided by the 1-Step System include:

  • Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range. Detects down to femtogram (fg) amounts of input RNA.
  • Exceptional room-temperature setup, allowing easy automation and high-throughput detection.



Linear dynamic range from 100ng down to 10fg of human RNA template. Seven 10-fold dilutions of human total RNA was run using 200nM of each GAPDH transcript specific primer. The amplification curve shows 97% efficiency and an r2=0.999.

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