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Convenient Master Mixes for Real-Time qPCR

• Ready-to-use mix of optimized components for dye-based qPCR
• Compatible with both fast and standard cycling methods
• Resistant to a wide range of PCR inhibitors

Sensitivity and Specificity for qPCR

The GoTaq® qPCR Master Mix is a ready-to-use 2X master mix for real-time quantitative PCR. Combining GoTaq® Hot Start Polymerase, optimized buffer and the BRYT Green® Dye, the GoTaq® qPCR Master Mix provides robust real-time PCR with earlier quantification cycle values and broad-range detection. These qualities result in increased reliability, reproducibility and sensitivity for quantitative, real-time PCR.

Designed for use with any instrument capable of reading SYBR® Green I or FAM™ Dye, the GoTaq® qPCR Master Mix offers:

• Sensitive detection for earlier quantitation of low- and high-copy targets.
• Room-temperature setup,making it suitable for automation and high-throughput detection.
• Compatibility with both fast and standard qPCR cycling methods.

qPCR with single-copy detection. The GAPDH gene was amplified and detected from 10-fold serial dilutions (0.01ng to 100ng) of human genomic DNA. Inset shows the standard curve for the various dilutions (Slope=-3.2; R2=0.995). Kit yield and purity were measured by absorbance spectroscopy.

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GoTaq(R) qPCR Master Mix Technical Manual TM318

GoTaq(R) qPCR Master Mix Quick Protocol #FB103