본문 바로가기 주메뉴 바로가기

인류의 건강과 생명공학 발전을 위해 봉사하는 기업 드림셀

We Serving the Health and Biotechnology of Humanity

제품정보

홈 > 제품정보 > 시약 > 국내 공식 대리점 > pGEM®-T Vector System I
제품명

pGEM®-T Vector System I

제품코드 : A3600

제품정보 : Cloning

  • PCR product를 cloning 하는데 유용한 제품입니다.

제품문의

  • 전화번호
    2292-8870

제품상세

Convenience for Cloning PCR Products

  • Insert excision with a BstZI single digest
  • Ligation can be completed in 1 hour at room temperature
  • Available with or without competent cells
The pGEM®-T Vector Systems are convenient systems to clone PCR products generated by certain thermostable polymerases. These polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments. The pGEM®-T Vector is ready to use in ligation reactions, prepared by cutting the pGEM®-5Zf(+) Vector with EcoRV and adding a 3´ terminal thymidine to both ends. These single 3´-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid by preventing recircularization of the vector and providing a compatible overhang for ligation of PCR products with A overhangs.

pGEM®-T Vector Map and Sequence



The pGEM®-T Vector is derived from the pGEM®-5Zf(+) Vector (GenBank® Accession No. X65308). The pGEM®-T Vector was created by linearizing the pGEM®-5Zf(+) Vector with EcoRV at base 51 and adding a T to both 3´-ends. The position of the T is indicated by * in the pGEM®-T Vector Sequence (.txt).


아래 링크를 클릭하시면 제품에 대한 자세한 정보를 확인하실 수 있습니다~