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홈 > 제품정보 > 시약 > 국내 공식 대리점 > GSH-Glo™ Glutathione Assay, 10ml
GSH-Glo™ Glutathione Assay, 10ml
제품코드 : V6911
제품정보 : Cell Stress Assay
- 세포 내에 남아있는 GSH를 측정하는 Luminescent-Based Assay 제품입니다.
- [V6911] 10ml, [V6912] 50ml
제품문의
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- 전화번호
- 2292-8870
- 이메일
- dreamcell@dreamcell.co.kr
제품상세
A Luminescent-Based Assay for the Detection and Quantification of Glutathione (GSH)
- Measure reduced (GSH) or total (GSSG + GSH) glutathione from cells and tissues
- Measure GSH levels directly from cell culture wells
- Excellent sensitivity and easily scalable
Sensitively Detect Total and Reduced GSH in Cells
The GSH-Glo™ Assay is a luminescent-based assay for the detection and quantification of glutathione (GSH) in cells or in various biological samples. A change in GSH levels is important when assessing toxicological responses and an indicator of oxidative stress, potentially leading to apoptosis or cell death. The GSH detection assay is based on the conversion of a luciferin derivative into luciferin in the presence of GSH. The reaction is catalyzed by a glutathione S-transferase (GST) enzyme supplied in the kit. The luciferin formed is detected in a coupled reaction using Ultra-Glo™ Recombinant Luciferase that generates a glow-type luminescence that is proportional to the amount of glutathione present in cells. The GSH detection assay provides a simple, fast and sensitive alternative to colorimetric and fluorescent methods and can be adapted easily to high-throughput applications.
Want to measure the ratio of reduced to oxidized glutathione?
The GSH/GSSG-Glo™ Assay is a luminescent assay that can quantify total glutathione (GSH + GSSG), GSSG and GSH-to-GSSG ratios in cultured cells. Learn more.
The GSH-Glo™ Glutathione Assay
The GSH assay is performed in two steps. In the first step, cells are lysed in the presence of the luciferin-NT substrate and glutathione S-transferase. Glutathione in the cells drives the formation of luciferin. In the 2nd step, Luciferin Detection Reagent is added to produce light that is directly proportional to the amount of GSH in the reaction.
GSH Depletion and Recovery in Treated Cells
HeLa cells (5000 cells/well in 96 well format) were exposed to l-Buthionine-sulfoximine (BSO). BSO inhibits GSH synthesis thus reducing cellular GSH level.
20hr BSO: Cells treated with BSO and assayed for GSH after 20 hours.
BSO + Recovery: Cells treated with BSO for 20 hours then washed 2X with PBS and covered with fresh media without BSO. Cells assayed for GSH after 40 hours.
40hr BSO: Cells treated with BSO, washed at 20 hours and fresh media plus BSO added. Cells assayed for GSH after 40 hours.
The simple two-reagent-addition GSH detection assay minimizes the number of assay steps compared to conventional GSH assays and is easily adapted to higher throughput applications. No deproteination step is required, and results are achieved in as quickly as 30 minutes.
The luminescent method of the GSH-Glo™ Glutathione Assay avoids inherent background fluorescence associated with other methods thereby providing excellent signal-to-background ratios. A half-life greater than 5 hours results in a stable signal, enabling batch processing of many plates at once using the GSH-Glo™ Assay.
The GSH-Glo™ Assay is recommended for measurement of total glutathione levels in samples. To measure the ratio, we recommend the GSH/GSSG-Glo™ Assay.