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Key benefits:

• Suitable for long-term routine culture of adherently growing established human cancer cell lines
• Compatible with most commonly used human cancer cell lines
• Serum- and xeno-free
• Standardized and reproducible system

Components:

• Cancer Cell Basal Medium
• Cancer Cell SupplementMix

Our Cancer Cell Line Medium XF is the working horse of the cancer cell culture toolbox. It allows you to cultivate or co-cultivate a range of different cancer cells, cancer-associated cells, and non-cancer cells such as immune or stromal cells. This rich growth medium supports the long-term cultivation of cancer cells over many passages.

You can use the Cancer Cell Line Medium XF as a standalone to expand and maintain a cancer cell population in a standardized and reproducible environment. Or you can use it in combination with the Primary Cancer Cell Culture System (PCCS) and the 3D Tumorsphere Medium XF, e.g., to establish cancer cell lines from tumor biopsies.

Due to the flexible nature of our Cancer Cell Line Medium XF, you can use it to support a broad range of cancer cell lines or cancer cells from tumor biopsies while maintaining the tumor-initiating cancer stem cells (CSCs).

Cancer Cell Line Medium XF is serum-free and xeno-free and provides an environment free from non-defined materials, such as fetal calf serum, extracts, or hydrolysates. Cancer Cell Line Medium XF also shows very low lot-to-lot variability.

These properties provide the ideal culture environment to be used for other applications such as co-cultivation of cancer cells and immune cells for cell therapy development like CAR-T cells. The serum- and xeno-free formulation supports the expansion of extracellular vesicle (EV)-producing cancer cells in an EV-free environment.

Note: The serum- and xeno-free formulation means non-availability of attachment factors. Hence, coating with an appropriate adhesion factor is a pre-requisite. For the establishment of the culture conditions, it is recommended to test fibronectin and vitronectin coating.

Cell types tested for serial passage with our Cancer Cell Line Medium XF: