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홈 > 제품정보 > 시약 > 해외 공식 대리점 > Recombinant Mouse Neurofilament light polypeptide(Nefl)
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Recombinant Mouse Neurofilament light polypeptide(Nefl)

제품코드 : CSB-YP015688MO

제품정보 : Others

  • Size: 500μg/100μg/20μg
  • Express system: Yeast
  • Species: Mus musculus (Mouse)
  • Tag Info: N-terminal 6xHis-tagged

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  • 전화번호
    2292-8870

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Product Details

Description
The gene fragment corresponding to the 2-543aa of the mouse Nefl protein was synthesized, with appropriate restriction sites suitable for in-frame cloning into an expression vector, with N-terminal 6xHis tag. The yeast was transfected with the expression vector, and the clone was expressed upon certain induction. After the induced cell centrifugation, the recombinant protein was purified from the cell extract and presented as N-terminal 6xHis-tagged fusion. This recombinant mouse Nefl protein's purity is greater than 90% assayed by SDS-PAGE. The Nefl protein ran to a band of about 50-66 kDa mo...Read more
PurityGreater than 90% as determined by SDS-PAGE.
Target NamesNefl
Uniprot No.P08551
Research AreaOthers
Alternative NamesNefl; Nf68; NflNeurofilament light polypeptide; NF-L; 68 kDa neurofilament protein; Neurofilament triplet L protein
SpeciesMus musculus (Mouse)
SourceYeast
Expression Region2-543aa
Target Protein SequenceSSFGYDPYFSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRSTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYSGLQSSSYLMSARSFPAYYTSHVQEEQTEVEETIEATKAEEAKDEPPSEGEAEEEEKEKEEGEEEEGAEEEEAAKDESEDTKEEEEGGEGEEEDTKESEEEEKKEESAGEEQVAKKKD
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight63.4kDa
Protein LengthFull Length of Mature Protein
Tag InfoN-terminal 6xHis-tagged
FormLiquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs		Protein FAQs
Storage ConditionStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf LifeThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead TimeDelivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
NotesRepeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COAPlease contact us to get it.

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