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홈 > 제품정보 > 시약 > 해외 공식 대리점 > Recombinant Mouse Apoptosis-associated speck-like protein containing a CARD(Pycard)
Recombinant Mouse Apoptosis-associated speck-like protein containing a CARD(Pycard)
제품코드 : CSB-EP861664MO
제품정보 : Cancer
- Size: 1mg/100μg/20μg
- Express system: E.coli
- Species: Mus musculus (Mouse)
- Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged
제품문의
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- 전화번호
- 2292-8870
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- dreamcell@dreamcell.co.kr
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Product Details
| Description | A cDNA sequence encoding full-length mouse apoptosis-associated speck-like protein containing a CARD (Pycard) was inserted a 10xHis--tag at the N-terminus and a Myc-tag at the C-terminus and then expressed in E.coli. The resulting protein consists of 1-193aa of mouse Pycard and has a calculated molecular weight of 28.9 kDa. Its purity is greater than 85% measured by SDS-PAGE. This recombinant Pycard protein may find uses in the specific antibody generation or the studies of Pycard-involving cancers. Pycard, also known as ASC, is an adaptor protein of the inflammasome complex which forms a...Read more |
| Purity | Greater than 85% as determined by SDS-PAGE. |
| Target Names | Pycard |
| Uniprot No. | Q9EPB4 |
| Research Area | Cancer |
| Alternative Names | Pycard; AscApoptosis-associated speck-like protein containing a CARD; mASC; PYD and CARD domain-containing protein |
| Species | Mus musculus (Mouse) |
| Source | E.coli |
| Expression Region | 1-193aa |
| Target Protein Sequence | MGRARDAILDALENLSGDELKKFKMKLLTVQLREGYGRIPRGALLQMDAIDLTDKLVSYYLESYGLELTMTVLRDMGLQELAEQLQTTKEESGAVAAAASVPAQSTARTGHFVDQHRQALIARVTEVDGVLDALHGSVLTEGQYQAVRAETTSQDKMRKLFSFVPSWNLTCKDSLLQALKEIHPYLVMDLEQS Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
| Mol. Weight | 28.9 kDa |
| Protein Length | Full Length |
| Tag Info | N-terminal 10xHis-tagged and C-terminal Myc-tagged |
| Form | Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs | Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | 3-7 business days |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |
Citations
- Mul1 suppresses Nlrp3 inflammasome activation through ubiquitination and degradation of Asc Kim J H, et al,Biorxiv,2019