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We Serving the Health and Biotechnology of Humanity
We Serving the Health and Biotechnology of Humanity
제품코드 : CSB-EP861191HU1
제품정보 : Transcription
Description | The production of this Recombinant Human FOXP3 protein required the insertion of a DNA fragment (FOXP3, 1-260aa) into an expression vector and the transferral of this vector into E.coli cells (the step of transformation). The cells were then cultured and induced to express the FOXP3 protein. This recombinant protein was fused with N-terminal 6xHis tag. Its purity is 90%+ determined by SDS-PAGE.FOXP3 (also called IPEX) is a gene encoding a protein called forkhead box protein P3 (FOXP3) (also known as scurfin) in human. The protein encoded by this gene can be cleaved into two chains, including f...Read more |
Purity | Greater than 90% as determined by SDS-PAGE. |
Target Names | FOXP3 |
Uniprot No. | Q9BZS1 |
Research Area | Transcription |
Alternative Names | AIID; DIETER; Forkhead box P3; Forkhead box protein P3; FOXP3; FOXP3_HUMAN; FOXP3delta7; Immune dysregulation polyendocrinopathy enteropathy X linked; Immunodeficiency polyendocrinopathy enteropathy X linked; IPEX; JM2; MGC141961; MGC141963; OTTHUMP00000025832; OTTHUMP00000025833; OTTHUMP00000226737; PIDX; Scurfin; XPID |
Species | Homo sapiens (Human) |
Source | E.coli |
Expression Region | 1-260aa |
Target Protein Sequence | MPNPRPGKPSAPSLALGPSPGASPSWRAAPKASDLLGARGPGGTFQGRDLRGGAHASSSSLNPMPPSQLQLPTLPLVMVAPSGARLGPLPHLQALLQDRPHFMHQLSTVDAHARTPVLQVHPLESPAMISLTPPTTATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPNPSAPRKDSTLSAVPQSSYPLLANGVCKWPGCEKVFEEPEDFLKHCQADHLLDEKGRAQCLLQREMVQSLEQQLVLEKEKLSAMQAHL Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
Mol. Weight | 31.7kDa |
Protein Length | Partial |
Tag Info | N-terminal 6xHis-tagged |
Form | Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Troubleshooting and FAQs | Protein FAQs |
Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
Lead Time | 3-7 business days |
Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Datasheet & COA | Please contact us to get it. |