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홈 > 제품정보 > 시약 > 해외 공식 대리점 > Recombinant Rat Semaphorin-3A(Sema3a)
Recombinant Rat Semaphorin-3A(Sema3a)
제품코드 : CSB-EP717391RA
제품정보 : Cardiovascular
- Size: 1mg/100μg/20μg
- Express system: E.coli
- Species: Rattus norvegicus (Rat)
- Tag Info: N-terminal 6xHis-tagged
제품문의
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- 전화번호
- 2292-8870
- 이메일
- dreamcell@dreamcell.co.kr
제품상세
Product Details
| Description | This protein is a recombinant Rat Sema3a protein with N-terminal 6xHis tag. Its purity is 90% determined by SDS-PAGE. The production includes 5 steps. 1, obtain the cDNA from the respective mRNA; 2, Select expression vector to insert the Sema3a sequence and get the recombinant plasmid of Sema3a; 3, Clone the recombinant plasmid into E.coli; 4, Express the Sema3a protein in the E.coli cell; 5, Protein purification. And finally, the desired recombinant protein was obtained. In addition, the activity has been tested.Semaphorin-3A is a protein encoded by the Sema3a gene. Semaphorin-3A is also cal...Read more |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Target Names | Sema3a |
| Uniprot No. | Q63548 |
| Research Area | Cardiovascular |
| Alternative Names | Sema3a; Semaphorin-3A; Semaphorin III; Sema III |
| Species | Rattus norvegicus (Rat) |
| Source | E.coli |
| Expression Region | 21-772aa |
| Target Protein Sequence | NYANGKNNVPRLKLSYKEMLESNNVITFNGLANSSSYHTFLLDEERSRLYVGAKDHIFSFNLVNIKDFQKIVWPVSYTRRDECKWAGKDILKECANFIKVLKAYNQTHLYACGTGAFHPICTYIEVGHHPEDNIFKLQDSHFENGRGKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHPIRTEQHDSRWLNDPRFISAHLIPESDNPEDDKVYFFFRENAIDGEHSGKATHARIGQICKNDFGGHRSLVNKWTTFLKARLICSVPGPNGIDTHFDELQDVFLMNSKDPKNPIVYGVFTTSSNIFKGSAVCMYSMSDVRRVFLGPYAHRDGPNYQWVPYQGRVPYPRPGTCPSKTFGGFDSTKDLPDDVITFARSHPAMYNPVFPINNRPIMIKTDVNYQFTQIVVDRVDAEDGQYDVMFIGTDVGTVLKVVSVPKETWHDLEEVLLEEMTVFREPTTISAMELSTKQQQLYIGSTAGVAQLPLHRCDIYGKACAECCLARDPYCAWDGSSCSRYFPTAKRRTRRQDIRNGDPLTHCSDLQHHDNHHGHSLEERIIYGVENSSTFLECSPKSQRALVYWQFQRRNEDRKEEIRVGDHIIRTEQGLLLRSLQKKDSGNYLCHAVEHGFMQTLLKVTLEVIDTEHLEELLHKDDDGDGSKTKEMSSSMTPSQKVWYRDFMQLINHPNLNTMDEFCEQVWKRDRKQRRQRPGHSQGSSNKWKHMQESKKGRNRRTHEFERAPRSV Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
| Mol. Weight | 90.6kDa |
| Protein Length | Full Length of Mature Protein |
| Tag Info | N-terminal 6xHis-tagged |
| Form | Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs | Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | 3-7 business days |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |