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홈 > 제품정보 > 시약 > 해외 공식 대리점 > Recombinant Human Acid ceramidase(ASAH1)
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Recombinant Human Acid ceramidase(ASAH1)

제품코드 : CSB-EP619774HU

제품정보 : Signal Transduction

  • Size: 1mg/100ug/20ug
  • Express system: E.coli
  • Species: Homo sapiens (Human)
  • Tag Info: N-terminal 6xHis-SUMO-tagged

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  • 전화번호
    2292-8870

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Product Details

Description
Recombinant Human Acid ceramidase (ASAH1) is a partial length protein expressed with N-terminal 6xHis-SUMO-tagged in the E.coli. Its expression region corresponds to 22-395aa of human ASAH1 protein. Its purity was determined by SDS-PAGE and reached up to 90% and presented a molecular mass band of 58.7 kDa on the gel. This recombinant ASAH1 protein may be used to synthesize antibodies against ASAH1. ASAH1 proteins in-stock are available. ASAH1 is lysosomal ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at acidic pH. Ceramides, sphingosine, and its phosp...Read more
PurityGreater than 90% as determined by SDS-PAGE.
Target NamesASAH1
Uniprot No.Q13510
Research AreaSignal Transduction
Alternative NamesAC; ACDase; Acid CDase; Acid ceramidase; Acid ceramidase precursor; Acid ceramidase subunit beta; Acylsphingosine deacylase; ASAH 1; ASAH; ASAH1; ASAH1_HUMAN; FLJ21558; FLJ22079; N acylsphingosine amidohydrolase (acid ceramidase) 1; N acylsphingosine amidohydrolase 1; N acylsphingosine amidohydrolase; N-acylsphingosine amidohydrolase; N-acylsphingosine deacylase; PHP; PHP32; Putative 32 kDa heart protein; SMAPME
SpeciesHomo sapiens (Human)
SourceE.coli
Expression Region22-395aa
Target Protein SequenceQHAPPWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight58.7kDa
Protein LengthFull Length of Mature Protein
Tag InfoN-terminal 6xHis-SUMO-tagged
FormLiquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs		Protein FAQs
Storage ConditionStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf LifeThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time3-7 business days
NotesRepeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COAPlease contact us to get it.

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