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> 제품정보 > 시약 > 해외 공식 대리점 > Recombinant Porphyromonas gingivalis Lys-gingipain(kgp),partial
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Recombinant Porphyromonas gingivalis Lys-gingipain(kgp),partial

제품코드 : CSB-EP464342EXZ

제품정보 : Others

  • Size: 1mg/100μg/20μg
  • Express system: E.coli
  • Species: Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / JCM 12257)
  • Tag Info: N-terminal 6xHis-SUMO-tagged

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  • 전화번호
    2292-8870

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Product Details

Description
A DNA fragment encoding the 229-594aa of Porphyromonas gingivalis Lys-gingipain (kgp) was fused with an N-terminal 6xHis-SUMO-tag and expressed in the E.coli. The resulting protein is the Recombinant partial-length Porphyromonas gingivalis kgp. Its purity (>90%) was determined by SDS-PAGE under reducing conditions and its component identity was validated by the LC-MS/MS analysis. This protein is used to produce specific antibodies or study issues-associated with chronic periodontitis. In-stock kgp proteins are available. Lysine-specific gingipain (kgp) is one type of gingipains in P. gingiv...Read more
PurityGreater than 90% as determined by SDS-PAGE.
Target Nameskgp
Uniprot No.B2RLK2
Research AreaOthers
Alternative Nameskgp; PGN_1728Lys-gingipain; EC 3.4.22.47; Lysine-specific cysteine proteinase Kgp) [Cleaved into: Lys-gingipain catalytic subunit; 39 kDa adhesin; 15 kDa adhesin; 44 kDa adhesin]
SpeciesPorphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / JCM 12257)
SourceE.coli
Expression Region229-594aa
Target Protein SequenceDVYTDHGDLYNTPVRMLVVAGAKFKEALKPWLTWKAQKGFYLDVHYTDEAEVGTTNASIKAFIHKKYNDGLAASAAPVFLALVGDTDVISGEKGKKTKKVTDLYYSAVDGDYFPEMYTFRMSASSPEELTNIIDKVLMYEKATMPDKSYLEKALLIAGADSYWNPKIGQQTIKYAVQYYYNQDHGYTDVYSYPKAPYTGCYSHLNTGVGFANYTAHGSETSWADPSLTATQVKALTNKDKYFLAIGNCCVTAQFDYPQPCFGEVMTRVKEKGAYAYIGSSPNSYWGEDYYWSVGANAVFGVQPTFEGTSMGSYDATFLEDSYNTVNSIMWAGNLAATHAGNIGNITHIGAHYYWEAYHVLGDGSVM
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight56.6kDa
Protein LengthPartial
Tag InfoN-terminal 6xHis-SUMO-tagged
FormLiquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage ConditionStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf LifeThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time3-7 business days
NotesRepeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COAPlease contact us to get it.