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홈 > 제품정보 > 시약 > 해외 공식 대리점 > Recombinant Streptococcus pneumoniae serotype 4 Pneumolysin(ply)
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Recombinant Streptococcus pneumoniae serotype 4 Pneumolysin(ply)

제품코드 : CSB-EP314690FMW

제품정보 : Others

  • Size: 1mg/100μg/20μg
  • Express system: E.coli
  • Species: Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
  • Tag Info: N-terminal 6xHis-SUMO-tagged

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  • 전화번호
    2292-8870

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Product Details

Description
Recombinant Streptococcus pneumoniae serotype 4 Pneumolysin (ply) is a partial-length protein expressed with an N-terminal 6xHis-SUMO-tagged in the E.coli. Its expression region corresponds to 2-471aa of Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) ply protein. This protein underwent validation via the LC-MS/MS analysis. Its purity was determined by SDS-PAGE and reached up to 90% and presented the mass molecule around 66.2 kDa was visualized on the gel. This recombinant ply protein may be used to synthesize antibodies against ply. Ply proteins in-stock are available. Ply ...Read more
PurityGreater than 90% as determined by SDS-PAGE.
Target Namesply
Uniprot No.P0C2J9
Research AreaOthers
Alternative Namesply; SP_1923; Pneumolysin; Ply; Thiol-activated cytolysin
SpeciesStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
SourceE.coli
Expression Region2-471aa
Target Protein SequenceANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight68.8kDa
Protein LengthFull Length of Mature Protein
Tag InfoN-terminal 6xHis-SUMO-tagged
FormLiquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs		Protein FAQs
Storage ConditionStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf LifeThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time3-7 business days
NotesRepeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COAPlease contact us to get it.

Citations

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