| Description | CUSABIO's SARS-CoV-2 Neutralizing Antibody ELISA Kit is intended for qualitative measurement of neutralizing antibodies in human serum or plasma. SARS-CoV-2 neutralizing antibodies directly target the SARS-CoV-2 S receptor-binding domain (RBD), blocking the protein's interaction with the host ACE2 receptor, thus interfering with the viral attachment to the cell as well as its entry into the cell. When using the reference neutralizing antibody provided in the kit, the assay has a detection range of 9.75-10000 ng/ml, a sensitivity of as low as 9.75 ng/ml, and Intra- /Inter-assay Precision low th...Read more |
| Alternative Names | 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2 |
| Abbreviation | SARS-CoV-2 neutralizing Ab |
| Species | Homo sapiens (Human) |
| Sample Types | serum, plasma |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Infectious Diseases
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| Assay Principle | qualitative |
| Measurement | Competitive |
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The assay employs the competitive inhibition enzyme immunoassay technique as well as a colorimetric reaction, in which the SARS-CoV-2 neutralizing antibodies in the sample and standard compete with the HRP-conjugated ACE2 to bind to the pre-coated SARS-CoV-2 S RBD. After the sequential addition of the TMB Substrate Solution and the Stop solution, the solution color develops in opposite to the amount of SARS-CoV-2 neutralizing antibodies in the sample. The presence of neutralizing antibodies in the sample can be detected by a reduction signal of HRP-ACE2/SARS-CoV-2 S RBD binding. |
| Precision | | Intra-assay Precision (Precision within an assay): CV%<15% | | Three samples of known concentration were tested twenty times on one plate to assess. | | Inter-assay Precision (Precision between assays): CV%<15% | | Three samples of known concentration were tested in twenty assays to assess. |
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| Typical Data | | These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| ng/ml | OD1 | OD2 | Average | | 10000 | 0.099 | 0.092 | 0.096 | | 5000 | 0.145 | 0.136 | 0.141 | | 2500 | 0.194 | 0.183 | 0.189 | | 1250 | 0.274 | 0.263 | 0.269 | | 625 | 0.344 | 0.371 | 0.358 | | 312 | 0.519 | 0.528 | 0.524 | | 156 | 0.901 | 0.907 | 0.904 | | 78 | 1.509 | 1.412 | 1.461 | | 39 | 1.787 | 1.723 | 1.755 | | 19.5 | 2.121 | 1.958 | 2.040 | | 9.75 | 2.233 | 2.138 | 2.186 | | 0 | 2.394 | 2.328 | 2.361 |
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| Materials provided | - One 96-well Microtiter plate ( coated Microwells) -- This microwell plate has been pre-coated with recombinant SARS-CoV-2 S RBD antigen and can be dismountable into 12 strips of 8 wells.
- A bottle of Standard (10 x concentrate; 200 μl/vial) -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
- A bottle of HRP-conjugated ACE2 (100 x concentrate; 60 μl/vial) -- ACE2 interacts with SARS-CoV-2 S RBD, mimicking the process of SARS-CoV-2 entry into the host cells. HRP catalyzes the TMB Substrate solution, eliciting a chromogenic reaction.
- A bottle of HRP-conjugate Diluent (10 ml/vial) -- Dilute the HRP-conjugated ACE2 solution.
- Two bottles of Sample Diluent (20 ml/vial) -- Reconstitute the standard and dilute the sample to an appropriate concentration.
- A bottle of Wash Buffer (25 x concentrate; 20 ml/vial) -- Wash away unbound or free substances.
- A bottle of TMB Substrate (10 ml/vial) -- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- A bottle of Stop Solution (10 ml/vial) -- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) -- Seal the plate when incubation.
- One Instruction manual
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| Materials not provided | - A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer.
- Absorbent paper for blotting the microtiter plate.
- 50-300ul multi-channel micropipette
- 100ml and 500ml graduated cylinders.
- Deionized or distilled water.
- Pipette tips
- Timer
- Test tubes for dilution.
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Troubleshooting
and FAQs | ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
