| Description | The product CSB-EL33241HU is an easy-to-use ELISA Kit intended for the qualitative detection of SARS-CoV-2 S1 RBD IgG antibody in human serum and plasma in vitro. SARS-CoV-2 S1 RBD IgG is an indicator of a recent or prior COVID infection. Infected patients develop IgG against SARS-CoV-2 S1 RBD 7 days post-infection, and IgG content peaks in the third week (IgM was close to vanishing at this time). IgG lasts in the blood for months or even years. After secondary infection, IgG levels in the body of patients increase rapidly and substantially in the short term and remain in the body for a long t...Read more |
| Alternative Names | S; 2; Spike glycoprotein; S glycoprotein; E2; Peplomer protein) |
| Abbreviation | SARS-CoV-2 S RBD Ab (IgG) |
| Uniprot No. | P0DTC2 |
| Species | Homo sapiens (Human) |
| Sample Types | serum, plasma |
| Detection Range | |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Infectious Diseases
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| Assay Principle | qualitative |
| Measurement | Indirect |
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The measurement of this assay is based on the indirect ELISA principle, in which the immune complex pre-coated antigen/S1 RBD IgG antibody/HRP-conjugated IgG is formed and then develops color reaction after the addition of TMB substrate solution. The color intensity is directly proportional to the amount of SARS-CoV-2 S1 RBD IgG antibody bound at the initial step. This kit serves as a tool to provide performance data during SARS-CoV-2-related research and development. The OD (optical density) of the sample below 2.1x negative OD was considered negative, and equal or above 2.1x negative OD was positive. It has high throughput with 90 samples at a time (6 wells used for the control) and has been validated with excellent specificity and high precision (less than 15%). |
| Precision | Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. |
| Typical Data | | Test parameter | specification | test result | | Positive control | ≥1.0 | 1.256 | | Negative control | ≤0.25 | 0.193 | | Positive rate | 20，Positive | 100% | | Negative rate | 20，Negative | 100% |
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| Materials provided | - A 96-well Coated assay plate 1 -- This microplate has been pre-coated with human SARS-CoV-2 S1 RBD antigen.
- Negative Control (1 x 800 μl) -- It is free of the SARS-CoV-2 S1 RBD IgG antibody and used to preclude the false positive.
- Positive Control (1 x 800 μl) -- Used to evaluate the validity, stability, and comparability of the test results.
- HRP-conjugated anti-Human IgG antibody (100 x concentrate) (1 x 120 μl) -- Act as the detection antibody.
- HRP-conjugate Diluent (1 x 20 ml) -- Dilute the HRPconjugated anti-Human IgG antibody.
- Sample Diluent (2 x 20 ml) -- Dilute the sample solution.
- Wash Buffer (25 x concentrate) (1 x 20 ml) -- Wash the unbound regeat.
- TMB Substrate (1 x 10 ml) -- React with HRP, eliciting a chromogenic color reaction.
- Stop Solution (1 x 10 ml) -- Stop the color reaction. The solution turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) -- Seal the microplate when incubation.
- An Instruction manual
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| Materials not provided | - A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer.
- Absorbent paper for blotting the microtiter plate.
- 50-300ul multi-channel micropipette
- 100ml and 500ml graduated cylinders.
- Deionized or distilled water.
- Pipette tips
- Timer
- Test tubes for dilution.
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Troubleshooting
and FAQs | ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
