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[CSB-E08542h] Human soluble Cluster of differentiation 86,sCD86 ELISA Kit

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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E08542h] Human soluble Cluster of differentiation 86,sCD86 ELISA Kit

제품코드 : CSB-E08542h

제품정보 : Human soluble Cluster of differentiation 86,sCD86

Sample Types: serum, plasma, tissue homogenates
Detect Range: 0.312 ng/mL-20 ng/mL
Sensitivity: 0.078 ng/mL

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Description

This Human CD86 ELISA Kit was designed for the quantitative measurement of Human CD86 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Target Name

CD86 molecule

Alternative Names

CD86; CD28LG2; T-lymphocyte activation antigen CD86; Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD antigen CD86

Abbreviation

CD86

Uniprot No.

P42081

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human sCD86 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	91-95
1:2	Average %	96
	Range %	91-101
1:4	Average %	99
	Range %	92-106
1:8	Average %	86
	Range %	82-90

Recovery

The recovery of human sCD86 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-95
EDTA plasma (n=4)	103	100-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.500	2.659	2.580	2.425
10	2.167	2.035	2.101	1.946
5	1.423	1.544	1.484	1.329
2.5	0.888	0.852	0.870	0.715
1.25	0.622	0.631	0.627	0.472
0.625	0.414	0.437	0.426	0.271
0.312	0.268	0.281	0.275	0.120
0	0.149	0.161	0.155