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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E15768h] Human Soluble Cluster of Differentiation 80 (sCD80) ELISA Kit

[CSB-E15768h] Human Soluble Cluster of Differentiation 80 (sCD80) ELISA Kit

제품코드 : CSB-E15768h

제품정보 : Human Soluble Cluster of Differentiation 80 (sCD80)

Sample Types: serum, plasma, cell culture supernates
Detect Range: 125 pg/mL-8000 pg/mL
Sensitivity: 31.25 pg/mL

제품문의

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

제품상세

Product Details

Description

This Human CD80 ELISA Kit was designed for the quantitative measurement of Human CD80 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.

Target Name

CD80 molecule

Alternative Names

CD80; CD28LG; CD28LG1; LAB7; T-lymphocyte activation antigen CD80; Activation B7-1 antigen; BB1; CTLA-4 counter-receptor B7.1; B7; CD antigen CD80

Abbreviation

CD80

Uniprot No.

P33681

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates

Detection Range

125 pg/mL-8000 pg/mL

Sensitivity

31.25 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human sCD80 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	99-107
1:2	Average %	87
	Range %	82-91
1:4	Average %	96
	Range %	92-100
1:8	Average %	90
	Range %	85-95

Recovery

The recovery of human sCD80 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-89
EDTA plasma (n=4)	94	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.618	2.485	2.552	2.435
4000	1.805	1.693	1.749	1.632
2000	1.004	1.047	1.026	0.909
1000	0.547	0.562	0.555	0.438
500	0.351	0.334	0.343	0.226
250	0.230	0.219	0.225	0.108
125	0.162	0.171	0.167	0.050
0	0.113	0.120	0.117