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> 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E14099h] Human cluster of differentiation 73(CD73) ELISA Kit
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[CSB-E14099h] Human cluster of differentiation 73(CD73) ELISA Kit

제품코드 : CSB-E14099h

제품정보 : Human cluster of differentiation 73(CD73)

  • Sample Types: serum, plasma, tissue homogenates
  • Detect Range: 3.12 ng/mL-200 ng/mL
  • Sensitivity: 0.78 ng/mL

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  • 전화번호
    2292-8870

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Product Details

Description

This Human CD73 ELISA Kit was designed for the quantitative measurement of Human CD73 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL .

Target Namecluster of differentiation 73(CD73)
Alternative Names5' NT ELISA Kit; 5' nucleotidase (CD73) ELISA Kit; 5' nucleotidase precursor ELISA Kit; 5' nucleotidase; ecto ELISA Kit; 5' nucleotidase; ecto (CD73) ELISA Kit; 5'-NT ELISA Kit; 5'-nucleotidase ELISA Kit; 5NTD_HUMAN ELISA Kit; CD73 ELISA Kit; CD73 antigen ELISA Kit; E5NT ELISA Kit; Ecto 5' nucleotidase ELISA Kit; Ecto-5'-nucleotidase ELISA Kit; eN ELISA Kit; eNT ELISA Kit; NT ELISA Kit; NT5 ELISA Kit; NT5E ELISA Kit; NTE ELISA Kit; Purine 5 Prime Nucleotidase ELISA Kit
AbbreviationCD73
Uniprot No.P21589
SpeciesHomo sapiens (Human)
Sample Typesserum, plasma, tissue homogenates
Detection Range3.12 ng/mL-200 ng/mL
Sensitivity0.78 ng/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD73 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %81-91
1:2Average %99
Range %93-103
1:4Average %99
Range %95-103
1:8Average %94
Range %88-98
Recovery
The recovery of human CD73 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)9692-98
EDTA plasma (n=4)9693-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2002.4982.4822.4902.363
1001.9321.8171.8751.748
501.2761.2561.2661.139
250.7270.7140.7210.594
12.50.4680.4440.4560.329
6.250.3430.3210.3320.205
3.120.2220.2120.2170.090
00.1290.1250.127 
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days