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[CSB-EL004955HU] Human B-cell differentiation antigen CD72(CD72) ELISA kit

제품코드 : CSB-EL004955HU

제품정보 : Human B-cell differentiation antigen CD72(CD72)

Sample Types: serum, plasma, tissue homogenates
Detect Range: 0.9 ng/mL-60 ng/mL
Sensitivity: 0.225 ng/mL

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전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

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Product Details

Description

This Human CD72 ELISA Kit was designed for the quantitative measurement of Human CD72 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 ng/mL-60 ng/mL and the sensitivity is 0.225 ng/mL.

Target Name

CD72 molecule

Alternative Names

CD72; B-cell differentiation antigen CD72; Lyb-2; CD antigen CD72

Abbreviation

CD72

Uniprot No.

P21854

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.9 ng/mL-60 ng/mL

Sensitivity

0.225 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CD72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-103
1:2	Average %	91
	Range %	86-98
1:4	Average %	92
	Range %	88-97
1:8	Average %	101
	Range %	96-15

Recovery

The recovery of human CD72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-95
EDTA plasma (n=4)	101	103-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
60	2.588	2.787	2.688	2.535
30	2.169	2.236	2.203	2.050
15	1.674	1.744	1.709	1.556
7.5	1.322	1.223	1.273	1.120
3.75	0.752	0.744	0.748	0.595
1.8	0.363	0.375	0.369	0.216
0.9	0.261	0.255	0.258	0.105
0	0.149	0.156	0.153

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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제품정보

[CSB-EL004955HU] Human B-cell differentiation antigen CD72(CD72) ELISA kit

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Product Details

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