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[CSB-EL004969HU] Human CD9 antigen(CD9) ELISA kit

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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-EL004969HU] Human CD9 antigen(CD9) ELISA kit

제품코드 : CSB-EL004969HU

제품정보 : Human CD9 antigen(CD9)

Sample Types: serum, plasma, tissue homogenates, cell lysates
Detect Range: 0.156 ng/mL-10 ng/mL
Sensitivity: 0.039 ng/mL

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Description

This Human CD9 ELISA Kit was designed for the quantitative measurement of Human CD9 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Target Name

CD9 molecule

Alternative Names

CD9; MIC3; TSPAN29; GIG2; CD9 antigen; 5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; MRP-1; Tetraspanin-29; Tspan-29; p24; CD antigen CD9

Abbreviation

CD9

Uniprot No.

P21926

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CD9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	81-90
1:2	Average %	96
	Range %	90-105
1:4	Average %	99
	Range %	91-110
1:8	Average %	92
	Range %	84-98

Recovery

The recovery of human CD9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	81-98
EDTA plasma (n=4)	92	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.209	2.152	2.181	2.030
5	1.710	1.706	1.708	1.557
2.5	1.244	1.204	1.224	1.073
1.25	0.860	0.839	0.850	0.699
0.625	0.493	0.459	0.476	0.325
0.312	0.320	0.295	0.308	0.157
0.156	0.241	0.229	0.235	0.084
0	0.154	0.147	0.151