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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-EL004959MO] Mouse T-lymphocyte activation antigen CD80(CD80) ELISA kit
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[CSB-EL004959MO] Mouse T-lymphocyte activation antigen CD80(CD80) ELISA kit

제품코드 : CSB-EL004959MO

제품정보 : Mouse T-lymphocyte activation antigen CD80(CD80)

  • Sample Types: serum, plasma, tissue homogenates
  • Detect Range: 15.6 pg/mL-1000 pg/mL
  • Sensitivity: 3.9 pg/mL

제품문의

  • 전화번호
    2292-8870

제품상세

Product Details

Target NameCD80 molecule
Alternative NamesCd80; B7; T-lymphocyte activation antigen CD80; Activation B7-1 antigen; CD antigen CD80
AbbreviationCD80
Uniprot No.Q00609
SpeciesMus musculus (Mouse)
Sample Typesserum, plasma, tissue homogenates
Detection Range15.6 pg/mL-1000 pg/mL
Sensitivity3.9 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Precision
Intra-assay Precision (Precision within an assay): CV%<8%    
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%    
Three samples of known concentration were tested in twenty assays to assess.   
        
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD80 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. 
　SampleSerum(n=4)  
1:1Average %94  
Range %91-98  
1:2Average %102  
Range %97-106  
1:4Average %85  
Range %81-89  
1:8Average %87  
Range %83-90  
Recovery
The recovery of mouse CD80 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. 
 
Sample TypeAverage % RecoveryRange  
Serum (n=5)10298-106  
EDTA plasma (n=4)8480-88  
        
        
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
 
pg/mlOD1OD2AverageCorrected  
25002.7932.7682.7812.614  
12502.2012.1062.1541.987  
6251.4631.5011.4821.315  
312.50.9050.9150.9100.743  
156.250.6060.6220.6140.447  
780.3670.3580.3630.196  
390.2830.2890.2860.119  
00.1660.1670.167　  
Troubleshooting
and FAQs		ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

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