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> 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E14007h] Human B-lymphocyte antigen (CD20) ELISA Kit
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[CSB-E14007h] Human B-lymphocyte antigen (CD20) ELISA Kit

제품코드 : CSB-E14007h

제품정보 : Human B-lymphocyte antigen (CD20)

  • Sample Types: serum, plasma, tissue homogenates
  • Detect Range: 3.12 ng/mL-200 ng/mL
  • Sensitivity: 0.78 ng/mL

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  • 전화번호
    2292-8870

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Product Details

Description

This Human MS4A1 ELISA Kit was designed for the quantitative measurement of Human MS4A1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL .

Target Namemembrane-spanning 4-domains, subfamily A, member 1
Alternative NamesMS4A1; CD20; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD antigen CD20
AbbreviationMS4A1
Uniprot No.P11836
SpeciesHomo sapiens (Human)
Sample Typesserum, plasma, tissue homogenates
Detection Range3.12 ng/mL-200 ng/mL
Sensitivity0.78 ng/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaCell Biology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD20 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %96
Range %90-100
1:2Average %89
Range %86-92
1:4Average %95
Range %90-99
1:8Average %90
Range %86-94
Recovery
The recovery of human CD20 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)9792-103
EDTA plasma (n=4)9589-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2001.8091.8491.8291.668
1001.5061.5191.5131.352
501.1291.2111.1701.009
250.8300.8460.8380.677
12.50.5630.5790.5710.410
6.250.3400.3540.3470.186
3.120.2430.2640.2540.093
00.1600.1620.161 
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days