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> 제품정보 > 시약 > 해외 공식 대리점 > [CSB-EL004956HU] Human HLA class II histocompatibility antigen gamma chain(CD74) ELISA kit
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[CSB-EL004956HU] Human HLA class II histocompatibility antigen gamma chain(CD74) ELISA kit

제품코드 : CSB-EL004956HU

제품정보 : Human HLA class II histocompatibility antigen gamma chain(CD74)

  • Sample Types: serum, plasma, tissue homogenates
  • Detect Range: 0.9 ng/mL-60 ng/mL
  • Sensitivity: 0.225 ng/mL

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  • 전화번호
    2292-8870

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Product Details

Description

This Human CD74 ELISA Kit was designed for the quantitative measurement of Human CD74 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 ng/mL-60 ng/mL and the sensitivity is 0.225 ng/mL.

Target NameCD74 molecule, major histocompatibility complex, class II invariant chain
Alternative NamesCD74; DHLAG; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; CD antigen CD74
AbbreviationCD74
Uniprot No.P04233
SpeciesHomo sapiens (Human)
Sample Typesserum, plasma, tissue homogenates
Detection Range0.9 ng/mL-60 ng/mL
Sensitivity0.225 ng/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD74 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %91
Range %86-94
1:2Average %97
Range %90-105
1:4Average %103
Range %97-108
1:8Average %89
Range %85-92
Recovery
The recovery of human CD74 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)9288-95
EDTA plasma (n=4)9790-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
602.3112.4802.3962.260
301.8651.9771.9211.785
151.5861.4821.5341.398
7.50.9991.0151.0070.871
3.750.6320.6140.6230.487
1.80.3740.3810.3780.242
0.90.2650.2470.2560.120
00.1340.1370.136
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days