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[CSB-E15979h] Human Cluster of Differentiation 68(CD68) ELISA kit

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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E15979h] Human Cluster of Differentiation 68(CD68) ELISA kit

제품코드 : CSB-E15979h

제품정보 : Human Cluster of Differentiation 68(CD68)

Sample Types: serum, plasma, tissue homogenates
Detect Range: 15.6 pg/mL-1000 pg/mL
Sensitivity: 3.9 pg/mL

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Description

This Human CD68 ELISA Kit was designed for the quantitative measurement of Human CD68 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

Target Name

CD68 molecule

Alternative Names

CD68; Macrosialin; Gp110; CD antigen CD68

Abbreviation

CD68

Uniprot No.

P34810

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CD68 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-95
1:2	Average %	97
	Range %	91-105
1:4	Average %	98
	Range %	90-104
1:8	Average %	93
	Range %	86-98

Recovery

The recovery of human CD68 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	92	85-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	1.859	1.893	1.876	1.665
500	1.556	1.567	1.562	1.351
250	1.179	1.198	1.189	0.978
125	0.880	0.951	0.916	0.705
62.5	0.613	0.623	0.618	0.407
31.2	0.390	0.412	0.401	0.190
15.6	0.293	0.304	0.299	0.088
0	0.210	0.211	0.211