| Description | This Human CD63 ELISA Kit was designed for the quantitative measurement of Human CD63 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL . |
| Target Name | CD63 molecule |
| Alternative Names | CD63; MLA1; TSPAN30; CD63 antigen; Granulophysin; Lysosomal-associated membrane protein 3; LAMP-3; Lysosome integral membrane protein 1; Limp1; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; Tetraspanin-30; Tspan-30; CD antigen CD63 |
| Abbreviation | CD63 |
| Uniprot No. | P08962 |
| Species | Homo sapiens (Human) |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 0.625 ng/mL-40 ng/mL |
| Sensitivity | 0.156 ng/mL |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology
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| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | | Intra-assay Precision (Precision within an assay): CV%<8% | | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | | Inter-assay Precision (Precision between assays): CV%<10% | | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | |
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| Linearity | | To assess the linearity of the assay, samples were spiked with high concentrations of human CD63 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | | Sample | Serum(n=4) | | | 1:1 | Average % | 96 | | | Range % | 92-104 | | | 1:2 | Average % | 87 | | | Range % | 81-94 | | | 1:4 | Average % | 98 | | | Range % | 92-106 | | | 1:8 | Average % | 96 | | | Range % | 92-103 | |
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| Recovery | | The recovery of human CD63 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
| Sample Type | Average % Recovery | Range | | | Serum (n=5) | 104 | 98-107 | | | EDTA plasma (n=4) | 93 | 89-103 | | | | | | | | | | | | | | | |
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| Typical Data | | These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 40 | 2.639 | 2.538 | 2.589 | 2.428 | | | 20 | 1.947 | 1.846 | 1.897 | 1.736 | | | 10 | 1.301 | 1.312 | 1.307 | 1.146 | | | 5 | 0.776 | 0.765 | 0.771 | 0.610 | | | 2.5 | 0.473 | 0.462 | 0.468 | 0.307 | | | 1.25 | 0.336 | 0.347 | 0.342 | 0.181 | | | 0.625 | 0.246 | 0.240 | 0.243 | 0.082 | | | 0 | 0.162 | 0.160 | 0.161 | | |
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| Materials provided | - A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human CD63 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled CD63 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
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| Materials not provided | - A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
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| ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs | ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
