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Product Details

[CSB-E11846h] Human cluster Of differentiation,CD44 ELISA Kit

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제품상세

홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E11846h] Human cluster Of differentiation,CD44 ELISA Kit

제품코드 : CSB-E11846h

제품정보 : Human cluster Of differentiation,CD44

Sample Types: serum, plasma, cell culture supernates, tissue homogenates
Detect Range: 78 pg/mL-5000 pg/mL
Sensitivity: 19.5 pg/mL

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Description

This Human CD44 ELISA Kit was designed for the quantitative measurement of Human CD44 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

Target Name

CD44 molecule (Indian blood group)

Alternative Names

LHR ELISA Kit; BA-1 ELISA Kit; CD 44 ELISA Kit; CD44 ELISA Kit; CD44 antigen ELISA Kit; CD44 molecule (Indian blood group) ELISA Kit; CD44 molecule ELISA Kit; CD44_HUMAN ELISA Kit; CDw44 ELISA Kit; CDW44 antigen ELISA Kit; Cell surface glycoprotein CD44 ELISA Kit; chondroitin sulfate proteoglycan 8 ELISA Kit; CSPG8 ELISA Kit; ECMR-III ELISA Kit; Epican ELISA Kit; Extracellular matrix receptor III ELISA Kit; GP90 lymphocyte homing/adhesion receptor ELISA Kit; HCELL ELISA Kit; hematopoietic cell E- and L-selectin ligand ELISA Kit; Heparan sulfate proteoglycan ELISA Kit; Hermes antigen ELISA Kit; homing function and Indian blood group system ELISA Kit; HSA ELISA Kit; HUTCH-I ELISA Kit; HUTCH1 ELISA Kit; HUTCHI ELISA Kit; Hyaluronate receptor ELISA Kit; IN ELISA Kit; INLU-related p80 Glycoprotein ELISA Kit; MC56 ELISA Kit; MDU2 ELISA Kit; MDU3 ELISA Kit; MGC10468 ELISA Kit; MIC4 ELISA Kit; MUTCH I ELISA Kit; MUTCH1 ELISA Kit; PGP-1 ELISA Kit; PGP-I ELISA Kit; PGP1 ELISA Kit; Phagocytic glycoprotein 1 ELISA Kit; Phagocytic glycoprotein I ELISA Kit; Soluble CD44 ELISA Kit

Abbreviation

CD44

Uniprot No.

P16070

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

78 pg/mL-5000 pg/mL

Sensitivity

19.5 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CD44 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	88
	Range %	80-92
1:200	Average %	98
	Range %	91-105
1:400	Average %	104
	Range %	92-110
1:800	Average %	94
	Range %	86-98

Recovery

The recovery of human CD44 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	96	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.749	2.733	2.741	2.629
2500	1.823	1.801	1.812	1.700
1250	1.072	1.044	1.058	0.946
625	0.599	0.562	0.581	0.469
312	0.375	0.354	0.365	0.253
156	0.241	0.235	0.238	0.126
78	0.181	0.169	0.175	0.063
0	0.116	0.108	0.112