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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E04717m] Mouse Soluble Cluster of differentiation 40 ligand,sCD40L ELISA Kit

[CSB-E04717m] Mouse Soluble Cluster of differentiation 40 ligand,sCD40L ELISA Kit

제품코드 : CSB-E04717m

제품정보 : Mouse Soluble Cluster of differentiation 40 ligand,sCD40L

Sample Types: serum, plasma, cell culture supernates, tissue homogenates
Detect Range: 0.47 ng/mL-30 ng/mL
Sensitivity: 0.117 ng/mL

제품문의

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

제품상세

Product Details

Description

This Mouse CD40LG ELISA Kit was designed for the quantitative measurement of Mouse CD40LG protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.47 ng/mL-30 ng/mL and the sensitivity is 0.117 ng/mL.

Target Name

CD40 ligand

Alternative Names

Cd40lg; Cd40l; Tnfsf5; CD40 ligand; CD40-L; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD antigen CD154

Abbreviation

CD40LG

Uniprot No.

P27548

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.47 ng/mL-30 ng/mL

Sensitivity

0.117 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse sCD40L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	84-92
1:2	Average %	103
	Range %	95-107
1:4	Average %	92
	Range %	87-98
1:8	Average %	92
	Range %	87-96

Recovery

The recovery of mouse sCD40L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-103
EDTA plasma (n=4)	104	96-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
30	2.732	2.694	2.713	2.581
15	2.194	2.085	2.140	2.008
7.5	1.476	1.436	1.456	1.324
3.75	0.953	0.963	0.958	0.826
1.875	0.533	0.523	0.528	0.396
0.94	0.367	0.354	0.361	0.229
0.47	0.263	0.257	0.260	0.128
0	0.133	0.131	0.132