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> 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E08956h] Human cluster of differentiation 4,CD4 ELISA Kit
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[CSB-E08956h] Human cluster of differentiation 4,CD4 ELISA Kit

제품코드 : CSB-E08956h

제품정보 : Human cluster of differentiation 4,CD4

  • Sample Types: serum, plasma
  • Detect Range: 0.625 ng/mL-40 ng/mL
  • Sensitivity: 0.156 ng/mL

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  • 전화번호
    2292-8870

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Product Details

Description

This Human CD4 ELISA Kit was designed for the quantitative measurement of Human CD4 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

Target NameCD4 molecule
Alternative NamesCD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD antigen CD4
AbbreviationCD4
Uniprot No.P01730
SpeciesHomo sapiens (Human)
Sample Typesserum, plasma
Detection Range0.625 ng/mL-40 ng/mL
Sensitivity0.156 ng/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%   
Three samples of known concentration were tested twenty times on one plate to assess. 
Inter-assay Precision (Precision between assays): CV%<10%   
Three samples of known concentration were tested in twenty assays to assess.  
       
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of Human CD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4) 
1:1Average %92 
Range %86-96 
1:2Average %101 
Range %95-105 
1:4Average %92 
Range %86-98 
1:8Average %98 
Range %94-102 
Recovery
The recovery of Human CD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange 
Serum (n=5)8783-95 
EDTA plasma (n=4)9692-102 
       
       
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected 
402.4852.5842.5352.362 
201.9282.0271.9781.805 
101.3651.2651.3151.142 
50.8620.8420.8520.679 
2.50.5640.5770.5710.398 
1.250.3790.3650.3720.199 
0.6250.2710.2630.2670.094 
00.1720.1730.173  
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days