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[CSB-E09296h] Human soluble cluster of differentiation 28,sCD28 ELISA Kit

제품코드 : CSB-E09296h

제품정보 : Human soluble cluster of differentiation 28,sCD28

Sample Types: serum, plasma, tissue homogenates
Detect Range: 0.625 ng/mL-40 ng/mL
Sensitivity: 0.156 ng/mL

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전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

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Product Details

Description

This Human CD28 ELISA Kit was designed for the quantitative measurement of Human CD28 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

Target Name

CD28 molecule

Alternative Names

CD28; T-cell-specific surface glycoprotein CD28; TP44; CD antigen CD28

Abbreviation

CD28

Uniprot No.

P10747

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.625 ng/mL-40 ng/mL

Sensitivity

0.156 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human sCD28 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	93-99
1:2	Average %	85
	Range %	81-89
1:4	Average %	94
	Range %	90-98
1:8	Average %	107
	Range %	103-110

Recovery

The recovery of human sCD28 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	102	98-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.260	2.302	2.281	2.158
20	1.743	1.702	1.723	1.600
10	1.227	1.304	1.266	1.143
5	0.851	0.832	0.842	0.719
2.5	0.504	0.498	0.501	0.378
1.25	0.311	0.301	0.306	0.183
0.625	0.206	0.200	0.203	0.080
0	0.121	0.124	0.123

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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제품정보

[CSB-E09296h] Human soluble cluster of differentiation 28,sCD28 ELISA Kit

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