본문 바로가기 주메뉴 바로가기

인류의 건강과 생명공학 발전을 위해 봉사하는 기업 드림셀

We Serving the Health and Biotechnology of Humanity

제품정보

> 제품정보 > 시약 > 해외 공식 대리점 > [CSB-EL004911MO] Mouse Programmed cell death 1 ligand 1(CD274) ELISA kit
제품명

[CSB-EL004911MO] Mouse Programmed cell death 1 ligand 1(CD274) ELISA kit

제품코드 : CSB-EL004911MO

제품정보 : Mouse Programmed cell death 1 ligand 1(CD274)

  • Sample Types: serum, plasma, tissue homogenates
  • Detect Range: 31.25 pg/mL-2000 pg/mL
  • Sensitivity: 7.81 pg/mL

제품문의

  • 전화번호
    2292-8870

제품상세

Product Details

Description

This Mouse CD274 ELISA Kit was designed for the quantitative measurement of Mouse CD274 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL .

Target NameCD274 molecule
Alternative NamesCd274 ELISA Kit; B7h1 ELISA Kit; Pdcd1l1 ELISA Kit; Pdcd1lg1 ELISA Kit; Pdl1Programmed cell death 1 ligand 1 ELISA Kit; PD-L1 ELISA Kit; PDCD1 ligand 1 ELISA Kit; Programmed death ligand 1 ELISA Kit; B7 homolog 1 ELISA Kit; B7-H1 ELISA Kit; CD antigen CD274 ELISA Kit
AbbreviationCD274
Uniprot No.Q9EP73
SpeciesMus musculus (Mouse)
Sample Typesserum, plasma, tissue homogenates
Detection Range31.25 pg/mL-2000 pg/mL
Sensitivity7.81 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaOthers
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%   
Three samples of known concentration were tested twenty times on one plate to assess. 
Inter-assay Precision (Precision between assays): CV%<10%   
Three samples of known concentration were tested in twenty assays to assess.  
       
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD274 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4) 
1:1Average %98 
Range %92-105 
1:2Average %94 
Range %88-97 
1:4Average %85 
Range %80-89 
1:8Average %88 
Range %83-95 
Recovery
The recovery of mouse CD274 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange 
Serum (n=5)9387-99 
EDTA plasma (n=4)9994-104 
       
       
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected 
20002.8052.6502.7282.560 
10002.4042.3752.3902.222 
5001.7091.8101.7601.592 
2500.9941.0081.0010.833 
1250.5950.5470.5710.403 
62.50.3720.3550.3640.196 
31.250.2570.2550.2560.088 
00.1710.1640.168  
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days