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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-EL004905MO] Mouse Endosialin(CD248) ELISA kit
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[CSB-EL004905MO] Mouse Endosialin(CD248) ELISA kit

제품코드 : CSB-EL004905MO

제품정보 : Mouse Endosialin(CD248)

  • Sample Types: serum, plasma, tissue homogenates
  • Detect Range: 7.81 pg/mL-500 pg/mL
  • Sensitivity: 1.95 pg/mL

제품문의

  • 전화번호
    2292-8870

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Product Details

Description

This Mouse CD248 ELISA Kit was designed for the quantitative measurement of Mouse CD248 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.81 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

Target NameCD248 molecule, endosialin
Alternative NamesCd248 ELISA Kit; Tem1 ELISA Kit; Endosialin ELISA Kit; Tumor endothelial marker 1 ELISA Kit; CD antigen CD248 ELISA Kit
AbbreviationCD248
Uniprot No.Q91V98
SpeciesMus musculus (Mouse)
Sample Typesserum, plasma, tissue homogenates
Detection Range7.81 pg/mL-500 pg/mL
Sensitivity1.95 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaOthers
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD248 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:100Average %95
Range %90-99
1:200Average %102
Range %96-107
1:400Average %93
Range %89-98
1:800Average %86
Range %80-92
Recovery
The recovery of mouse CD248 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)9793-102
EDTA plasma (n=4)9894-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
5002.6432.7812.7122.625
2501.7651.8221.7941.707
1251.1211.2111.1661.079
62.50.7210.7100.7160.629
31.250.4330.4480.4410.354
15.620.2760.2820.2790.192
7.810.1820.1880.1850.098
00.0860.0880.087　
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

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