| Description | This Human CD163 ELISA Kit was designed for the quantitative measurement of Human CD163 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL. |
| Target Name | CD163 molecule |
| Alternative Names | C163A_HUMAN ELISA Kit; CD 163 ELISA Kit; CD163 ELISA Kit; CD163 antigen ELISA Kit; CD163 molecule ELISA Kit; Hemoglobin scavenger receptor ELISA Kit; M130 ELISA Kit; M130 antigen precursor ELISA Kit; Macrophage associated antigen ELISA Kit; MM130 ELISA Kit; OTTHUMP00000238617 ELISA Kit; OTTHUMP00000238618 ELISA Kit; OTTHUMP00000238619 ELISA Kit; OTTHUMP00000238620 ELISA Kit; SCARI1 ELISA Kit; Scavenger receptor cysteine rich type 1 protein M130 ELISA Kit; sCD163 ELISA Kit; Soluble CD163 ELISA Kit |
| Abbreviation | CD163 |
| Uniprot No. | Q86VB7 |
| Species | Homo sapiens (Human) |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 1.56 ng/mL-100 ng/mL |
| Sensitivity | 0.39 ng/mL |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology
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| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | | Intra-assay Precision (Precision within an assay): CV%<8% | | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | | Inter-assay Precision (Precision between assays): CV%<10% | | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | |
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| Linearity | | To assess the linearity of the assay, samples were spiked with high concentrations of human sCD163 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | | Sample | Serum(n=4) | | | 1:5 | Average % | 88 | | | Range % | 83-93 | | | 1:10 | Average % | 95 | | | Range % | 89-100 | | | 1:20 | Average % | 97 | | | Range % | 92-103 | | | 1:40 | Average % | 95 | | | Range % | 91-99 | |
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| Recovery | | The recovery of human sCD163 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
| Sample Type | Average % Recovery | Range | | | Serum (n=5) | 95 | 89-99 | | | EDTA plasma (n=4) | 98 | 93-102 | | | | | | | | | | | | | | | |
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| Typical Data | | These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.452 | 2.552 | 2.502 | 2.368 | | | 50 | 2.134 | 2.176 | 2.155 | 2.021 | | | 25 | 1.539 | 1.497 | 1.518 | 1.384 | | | 12.5 | 0.728 | 0.739 | 0.734 | 0.600 | | | 6.25 | 0.412 | 0.424 | 0.418 | 0.284 | | | 3.12 | 0.229 | 0.239 | 0.234 | 0.100 | | | 1.56 | 0.194 | 0.201 | 0.198 | 0.064 | | | 0 | 0.132 | 0.135 | 0.134 | | |
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| Materials provided | - A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human CD163 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled CD163 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
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| Materials not provided | - A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
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| ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs | ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
