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> 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E11178r] Rat soluble cluster of differentiation 14,sCD14 ELISA Kit
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[CSB-E11178r] Rat soluble cluster of differentiation 14,sCD14 ELISA Kit

제품코드 : CSB-E11178r

제품정보 : Rat soluble cluster of differentiation 14,sCD14

  • Sample Types: serum, plasma, tissue homogenates
  • Detect Range: 28 ng/mL-1800 ng/mL
  • Sensitivity: 7 ng/mL

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  • 전화번호
    2292-8870

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Product Details

Description

This Rat CD14 ELISA Kit was designed for the quantitative measurement of Rat CD14 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 28 ng/mL-1800 ng/mL and the sensitivity is 7 ng/mL.

Target NameCD14 molecule
Alternative NamesCd14Monocyte differentiation antigen CD14 ELISA Kit; Myeloid cell-specific leucine-rich glycoprotein ELISA Kit; CD antigen CD14 ELISA Kit
AbbreviationCD14
Uniprot No.Q63691
SpeciesRattus norvegicus (Rat)
Sample Typesserum, plasma, tissue homogenates
Detection Range28 ng/mL-1800 ng/mL
Sensitivity7 ng/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat sCD14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %86-100
1:2Average %99
Range %90-103
1:4Average %89
Range %83-92
1:8Average %95
Range %84-98
Recovery
The recovery of rat sCD14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)10294-108
EDTA plasma (n=4)9689-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
18002.5302.4012.4662.283
9002.0661.9862.0261.843
4501.3111.3561.3341.151
2250.7590.7790.7690.586
112.50.4560.4780.4670.284
560.3520.3440.3480.165
280.2710.2630.2670.084
00.1810.1850.183
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days