| Description | This Rat VCAM1 ELISA Kit was designed for the quantitative measurement of Rat VCAM1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL. |
| Target Name | vascular cell adhesion protein 1 (VCAM-1/CD106) |
| Alternative Names | Vcam1 ELISA Kit; Vcam-1 ELISA Kit; Vascular cell adhesion protein 1 ELISA Kit; V-CAM 1 ELISA Kit; VCAM-1 ELISA Kit; CD antigen CD106 ELISA Kit |
| Abbreviation | VCAM1 |
| Uniprot No. | P29534 |
| Species | Rattus norvegicus (Rat) |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 15.6 ng/mL-1000 ng/mL |
| Sensitivity | 3.9 ng/mL |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction
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| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | | Intra-assay Precision (Precision within an assay): CV%<8% | | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | | Inter-assay Precision (Precision between assays): CV%<10% | | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | | |
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| Linearity | | To assess the linearity of the assay, samples were spiked with high concentrations of rat VCAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | | Sample | Serum(n=4) | | | 1:1 | Average % | 89 | | | Range % | 85-94 | | | 1:2 | Average % | 95 | | | Range % | 86-103 | | | 1:4 | Average % | 96 | | | Range % | 82-105 | | | 1:8 | Average % | 98 | | | Range % | 87-105 | |
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| Recovery | | The recovery of rat VCAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
| Sample Type | Average % Recovery | Range | | | Serum (n=5) | 95 | 89-104 | | | EDTA plasma (n=4) | 89 | 85-93 | | | | | | | | | | | | | | | | | |
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| Typical Data | | These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.614 | 2.704 | 2.659 | 2.525 | | | 500 | 2.145 | 2.236 | 2.191 | 2.057 | | | 250 | 1.516 | 1.536 | 1.526 | 1.392 | | | 125 | 0.863 | 0.848 | 0.856 | 0.722 | | | 62.5 | 0.571 | 0.549 | 0.560 | 0.426 | | | 31.25 | 0.352 | 0.347 | 0.350 | 0.216 | | | 15.6 | 0.234 | 0.244 | 0.239 | 0.105 | | | 0 | 0.135 | 0.132 | 0.134 | | |
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| Materials provided | - A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat VCAM antibody.This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled VCAM antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
- One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
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| Materials not provided | - A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
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| ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs | ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
