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[CSB-E13037h] Human cluster Of differentiation 82 (CD82) ELISA Kit

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제품상세

홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E13037h] Human cluster Of differentiation 82 (CD82) ELISA Kit

제품코드 : CSB-E13037h

제품정보 : Human cluster Of differentiation 82 (CD82)

Sample Types: serum, plasma
Detect Range: 0.156 ng/mL-10 ng/mL
Sensitivity: 0.039 ng/mL

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Description

This Human CD82 ELISA Kit was designed for the quantitative measurement of Human CD82 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Target Name

CD82 molecule

Alternative Names

CD82; KAI1; SAR2; ST6; TSPAN27; CD82 antigen; C33 antigen; IA4; Inducible membrane protein R2; Metastasis suppressor Kangai-1; Suppressor of tumorigenicity 6 protein; Tetraspanin-27; Tspan-27; CD antigen CD82

Abbreviation

CD82

Uniprot No.

P27701

Species

Homo sapiens (Human)

Sample Types

serum, plasma

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CD82 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-98
1:2	Average %	97
	Range %	92-101
1:4	Average %	95
	Range %	90-99
1:8	Average %	84
	Range %	80-88

Recovery

The recovery of human CD82 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	99-110
EDTA plasma (n=4)	89	82-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.748	2.702	2.725	2.601
5	2.131	2.192	2.162	2.038
2.5	1.491	1.416	1.454	1.330
1.25	0.866	0.827	0.847	0.723
0.625	0.546	0.502	0.524	0.400
0.312	0.363	0.391	0.377	0.253
0.156	0.254	0.239	0.247	0.123
0	0.125	0.123	0.124