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> 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E07395r] Rat Soluble Cluster of differentiation 40 ligand,sCD40L ELISA Kit
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[CSB-E07395r] Rat Soluble Cluster of differentiation 40 ligand,sCD40L ELISA Kit

제품코드 : CSB-E07395r

제품정보 : Rat Soluble Cluster of differentiation 40 ligand,sCD40L

  • Sample Types: serum, plasma, tissue homogenates
  • Detect Range: 1.87 ng/mL-120 ng/mL
  • Sensitivity: 0.47 ng/mL

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  • 전화번호
    2292-8870

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Product Details

Description

This Rat CD40LG ELISA Kit was designed for the quantitative measurement of Rat CD40LG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.87 ng/mL-120 ng/mL and the sensitivity is 0.47 ng/mL.

Target NameCD40 ligand
Alternative NamesCd40lg ELISA Kit; Cd40l ELISA Kit; Tnfsf5CD40 ligand ELISA Kit; CD40-L ELISA Kit; Tumor necrosis factor ligand superfamily member 5 ELISA Kit; CD antigen CD154) [Cleaved into: CD40 ligand ELISA Kit; membrane form; CD40 ligand ELISA Kit; soluble form ELISA Kit; sCD40L)] ELISA Kit
AbbreviationCD40LG
Uniprot No.Q9Z2V2
SpeciesRattus norvegicus (Rat)
Sample Typesserum, plasma, tissue homogenates
Detection Range1.87 ng/mL-120 ng/mL
Sensitivity0.47 ng/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat sCD40L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %95
Range %83-98
1:2Average %93
Range %87-98
1:4Average %91
Range %83-95
1:8Average %103
Range %95-107
Recovery
The recovery of rat sCD40L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)8882-92
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
1202.4872.6882.5882.437
601.7341.6431.6891.538
301.0491.1061.0780.927
150.6740.6850.6800.529
7.50.4420.4570.4500.299
3.750.3140.3270.3210.170
1.870.2360.2450.2410.090
00.1500.1510.151
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days