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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E12928r] Rat cluster of differentiation 4,CD4 ELISA Kit

[CSB-E12928r] Rat cluster of differentiation 4,CD4 ELISA Kit

제품코드 : CSB-E12928r

제품정보 : Rat cluster of differentiation 4,CD4

Sample Types: serum, plasma, tissue homogenates
Detect Range: 0.78 U/mL-50 U/mL
Sensitivity: 0.195 U/mL

제품문의

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

제품상세

Product Details

Description

This Rat CD4 ELISA Kit was designed for the quantitative measurement of Rat CD4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 U/mL-50 U/mL and the sensitivity is 0.195 U/mL.

Target Name

CD4 molecule

Alternative Names

Cd4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; W3/25 antigen; CD antigen CD4

Abbreviation

CD4

Uniprot No.

P05540

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.78 U/mL-50 U/mL

Sensitivity

0.195 U/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	87-102
1:2	Average %	101
	Range %	94-105
1:4	Average %	99
	Range %	88-103
1:8	Average %	105
	Range %	98-109

Recovery

The recovery of rat CD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-98
EDTA plasma (n=4)	98	89-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

U/ml	OD1	OD2	Average	Corrected
50	2.656	2.587	2.622	2.508
25	2.171	2.062	2.117	2.003
12.5	1.438	1.388	1.413	1.299
6.25	0.720	0.715	0.718	0.604
3.12	0.412	0.424	0.418	0.304
1.56	0.261	0.252	0.257	0.143
0.78	0.176	0.183	0.180	0.066
0	0.112	0.115	0.114