| Description | This ELISA kit is used for quantitative detection of a human soluble cluster of differentiation 14 (sCD14), establishing a normal detection range and validation of antibody array results. CD14 is a pattern-recognition receptor and exists in two different forms: GPI-anchored membrane CD14 (mCD14) and liver-derived serum soluble CD14. sCD14 is an acute-phase protein upregulated significantly during sepsis but is also found in normal serum at microgram concentrations. CD14 plays an important role in the phagocytic clearance of apoptotic cells and the reactivation and immune recognition of microbi...Read more |
| Target Name | CD14 molecule |
| Alternative Names | CD 14 ELISA Kit; CD_antigen=CD14 ELISA Kit; CD14 ELISA Kit; CD14 antigen ELISA Kit; CD14 molecule ELISA Kit; CD14_HUMAN ELISA Kit; LPS-R ELISA Kit; Mo2 ELISA Kit; Monocyte differentiation antigen CD14 ELISA Kit; Monocyte differentiation antigen CD14 urinary form ELISA Kit; Monocyte differentiation antigen CD14; membrane-bound form ELISA Kit; Myeloid cell specific leucine rich glycoprotein ELISA Kit; Myeloid cell-specific leucine-rich glycoprotein ELISA Kit |
| Abbreviation | CD14 |
| Uniprot No. | P08571 |
| Species | Homo sapiens (Human) |
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates, urine |
| Detection Range | 0.234 ng/mL-15 ng/mL |
| Sensitivity | 0.229 ng/mL |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology
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| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays):CV%<10% Three samples of known concentration were tested in twenty assays to assess. 
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| Linearity | To assess the linearity of the assay, samples were spiked with high concentrations of human sCD14in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. 
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| Typical Data | These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
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| Materials provided | - A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human CD14 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled CD14 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
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| Materials not provided | - A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
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| ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs | ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
