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[CSB-E11987r] Rat TNF-α ELISA kit

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제품상세

홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E11987r] Rat TNF-α ELISA kit

제품코드 : CSB-E11987r

제품정보 : Rat TNF-α

Sample Types: serum, plasma, cell culture supernates, tissue homogenates
Detect Range: 6.25 pg/mL-400 pg/mL
Sensitivity: 1.56 pg/mL

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Description

This Rat TNF ELISA Kit was designed for the quantitative measurement of Rat TNF protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL .

Target Name

tumor necrosis factor (TNF superfamily, member 2)

Alternative Names

Tnf ELISA Kit; Tnfa ELISA Kit; Tnfsf2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit

Abbreviation

TNF

Uniprot No.

P16599

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

6.25 pg/mL-400 pg/mL

Sensitivity

1.56 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-96
1:2	Average %	99
	Range %	95-104
1:4	Average %	101
	Range %	97-105
1:8	Average %	98
	Range %	93-107

Recovery

The recovery of rat TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-92
EDTA plasma (n=4)	99	95-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.037	2.067	2.052	1.871
200	1.445	1.489	1.467	1.286
100	1.017	1.034	1.026	0.845
50	0.655	0.677	0.666	0.485
25	0.411	0.438	0.425	0.244
12.5	0.351	0.378	0.365	0.184
6.25	0.297	0.282	0.290	0.109
0	0.183	0.179	0.181