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[CSB-E04744m] Mouse Tumor necrosis factor β,TNF-β ELISA KIT

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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E04744m] Mouse Tumor necrosis factor β,TNF-β ELISA KIT

제품코드 : CSB-E04744m

제품정보 : Mouse Tumor necrosis factor β,TNF-β

Sample Types: serum, plasma, tissue homogenates
Detect Range: 18.75 pg/mL-1200 pg/mL
Sensitivity: 4.7 pg/mL

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Description

This Mouse LTA ELISA Kit was designed for the quantitative measurement of Mouse LTA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.7 pg/mL.

Target Name

lymphotoxin alpha (TNF superfamily, member 1)

Alternative Names

Lta ELISA Kit; Tnfb ELISA Kit; Tnfsf1 ELISA Kit; Lymphotoxin-alpha ELISA Kit; LT-alpha ELISA Kit; TNF-beta ELISA Kit; Tumor necrosis factor ligand superfamily member 1 ELISA Kit

Abbreviation

LTA

Uniprot No.

P09225

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

18.75 pg/mL-1200 pg/mL

Sensitivity

4.7 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNF-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-100
1:2	Average %	105
	Range %	100-110
1:4	Average %	92
	Range %	88-100
1:8	Average %	84
	Range %	80-88

Recovery

The recovery of mouse TNF-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	90	85-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.674	2.752	2.713	2.534
600	2.254	2.176	2.215	2.036
300	1.435	1.404	1.420	1.241
150	0.805	0.839	0.822	0.643
75	0.498	0.524	0.511	0.332
37.5	0.356	0.338	0.347	0.168
18.75	0.254	0.239	0.247	0.068
0	0.175	0.182	0.179