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[CSB-E04743h] Human Tumor necrosis factor β,TNF-β ELISA KIT

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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E04743h] Human Tumor necrosis factor β,TNF-β ELISA KIT

제품코드 : CSB-E04743h

제품정보 : Human Tumor necrosis factor β,TNF-β

Sample Types: serum, plasma, cell culture supernates
Detect Range: 15.6 pg/mL-1000 pg/mL
Sensitivity: 3.9 pg/mL

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Description

This Human LTA ELISA Kit was designed for the quantitative measurement of Human LTA protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Target Name

lymphotoxin alpha (TNF superfamily, member 1)

Alternative Names

LTalpha ELISA Kit; DAMA-25N12.13-004 ELISA Kit; DIF ELISA Kit; LT alpha ELISA Kit; LT ELISA Kit; LT-alpha ELISA Kit; Lta ELISA Kit; Lymphotoxin alpha (TNF superfamily, member 1) ELISA Kit; Lymphotoxin alpha ELISA Kit; Lymphotoxin-alpha ELISA Kit; MGC117668 ELISA Kit; OTTHUMP00000037612 ELISA Kit; OTTHUMP00000037613 ELISA Kit; TNF B ELISA Kit; TNF superfamily member 1 ELISA Kit; TNF, lymphocyte-derived ELISA Kit; TNF-beta ELISA Kit; TNFB ELISA Kit; TNFB_HUMAN ELISA Kit; TNFbeta ELISA Kit; TNFSF 1 ELISA Kit; TNFSF1 ELISA Kit; TNLG1E ELISA Kit; Tumor necrosis factor beta ELISA Kit; tumor necrosis factor ligand 1E ELISA Kit; Tumor necrosis factor ligand superfamily member 1 ELISA Kit; tumor necrosis factor superfamily member 1 ELISA Kit

Abbreviation

LTA

Uniprot No.

P01374

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TNF-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	83-99
1:2	Average %	87
	Range %	84-93
1:4	Average %	96
	Range %	90-104
1:8	Average %	93
	Range %	86-102

Recovery

The recovery of human TNF-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-100
EDTA plasma (n=4)	97	90-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	1.823	1.911	1.867	1.779
500	1.315	1.376	1.346	1.258
250	0.807	0.816	0.812	0.724
125	0.533	0.573	0.553	0.465
62.5	0.324	0.308	0.316	0.228
31.2	0.259	0.246	0.253	0.165
15.6	0.159	0.163	0.161	0.073
0	0.086	0.090	0.088