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[CSB-E04740h] Human Tumor necrosis factor α,TNF-α ELISA KIT

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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E04740h] Human Tumor necrosis factor α,TNF-α ELISA KIT

제품코드 : CSB-E04740h

제품정보 : Human Tumor necrosis factor α,TNF-α

Sample Types: serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detect Range: 7.8 pg/mL-500 pg/mL
Sensitivity: 1.95 pg/mL

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Description

Human TNF-α ELISA Kit is designed to quantify human TNF-α levels in the serum, plasma, cell culture supernates, tissue homogenates, or cell lysates. It quantitates human TNF-α with 1.95 pg/mL sensitivity and shows excellent specificity for human TNF-α. This human TNF-α ELISA kit includes both the capture and the detection antibodies for TNF-α as well as avidin conjugated Horseradish Peroxidase (HRP) and TMB substrate solution, which are the reagents used in color development step. The produced color develops in proportion to the amount of TNF-α in the sample. The color development is stopped a...Read more

Target Name

tumor necrosis factor (TNF superfamily, member 2)

Alternative Names

APC1 ELISA Kit; APC1 protein ELISA Kit; Cachectin ELISA Kit; DIF ELISA Kit; Differentiation inducing factor ELISA Kit; Macrophage cytotoxic factor ELISA Kit; Tnf ELISA Kit; TNF superfamily member 2 ELISA Kit; TNF superfamily, member 2 ELISA Kit; TNF, macrophage derived ELISA Kit; TNF, monocyte derived ELISA Kit; TNF-a ELISA Kit; TNF-alpha ELISA Kit; TNFA ELISA Kit; TNFA_HUMAN ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor (TNF superfamily member 2) ELISA Kit; Tumor necrosis factor alpha ELISA Kit; Tumor necrosis factor ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; Tumor Necrosis Factor, Membrane Form ELISA Kit; Tumor necrosis factor, soluble form ELISA Kit

Abbreviation

TNF

Uniprot No.

P01375

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates, cell lysates

Detection Range

7.8 pg/mL-500 pg/mL

Sensitivity

1.95 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	89-99
1:2	Average %	90
	Range %	85-95
1:4	Average %	97
	Range %	88-102
1:8	Average %	92
	Range %	85-100

Recovery

The recovery of human TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	94-101
EDTA plasma (n=4)	96	88-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.607	2.705	2.656	2.482
250	2.296	2.307	2.302	2.128
125	1.642	1.624	1.633	1.459
62.5	0.912	0.940	0.926	0.752
31.2	0.489	0.504	0.497	0.323
15.6	0.317	0.328	0.323	0.149
7.8	0.282	0.271	0.277	0.103
0	0.172	0.175	0.174