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[CSB-E13145m] Mouse Interleukin 35(IL-35) ELISA Kit

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제품상세

홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E13145m] Mouse Interleukin 35(IL-35) ELISA Kit

제품코드 : CSB-E13145m

제품정보 : Mouse Interleukin 35(IL-35)

Sample Types: serum, plasma, cell culture supernates, tissue homogenates
Detect Range: 15.6 pg/mL-1000 pg/mL
Sensitivity: 3.9 pg/mL

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

Description

This Mouse IL-35 ELISA Kit was designed for the quantitative measurement of Mouse IL-35 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Target Name

Interleukin 35(IL-35)

Abbreviation

IL-35

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	87-97
1:2	Average %	95
	Range %	89-99
1:4	Average %	94
	Range %	90-100
1:8	Average %	93
	Range %	87-97

Recovery

The recovery of mouse IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-98
EDTA plasma (n=4)	96	91-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.461	2.391	2.426	2.235
500	2.073	1.965	2.019	1.828
250	1.669	1.577	1.623	1.432
125	1.272	1.183	1.228	1.037
62.5	0.814	0.804	0.809	0.618
31.2	0.567	0.525	0.546	0.355
15.6	0.335	0.314	0.325	0.134
0	0.194	0.188	0.191

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Immunomodulatory activity-guided isolation and characterization of a novel polysaccharide from Atractylodis macrocephalae Koidz W Xue,International Journal of Biological Macromolecules,2020
Human placental trophoblast cells contribute to maternal-fetal tolerance through expressing IL-35 and mediating iTR35 conversion Liu J, et al,Nature Communications,2019
Interleukin-35 expression protects against cigarette smoke-induced lung inflammation in mice Pan X, et al,Biomedicine & pharmacotherapy,2018
Administration of Interleukin-35-Conditioned Autologous Tolerogenic Dendritic Cells Prolong Allograft Survival After Heart Transplantation Liu X.et al,Cell Physiol Biochem,2018
Imbalance of Th17/Treg in the Pathogenesis of Mice with Paraquat-induced Acute Lung Injury Xia Yang.et al,Iranian Journal of Allergy, Asthma and Immunology,2017
Isorhamnetin attenuates collagen-induced arthritis via modulating cytokines and oxidative stress in mice Wang X. et al,Int J Clin Exp Med,2015
The role of CCR7 in allergic airway inflammation induced by house dust mite exposure /,Cellular Immunology,2012

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.