| Description | This Human IL-35 ELISA Kit was designed for the quantitative measurement of Human IL-35 protein in serum, cell culture supernates, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL. |
| Target Name | Interleukin 35(IL-35) |
| Abbreviation | IL-35 |
| Species | Homo sapiens (Human) |
| Sample Types | serum, cell culture supernates, urine, tissue homogenates |
| Detection Range | 62.5 pg/mL-4000 pg/mL |
| Sensitivity | 15.6 pg/mL |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Others
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| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | | Intra-assay Precision (Precision within an assay): CV%<8% | | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | | Inter-assay Precision (Precision between assays): CV%<10% | | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | | |
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| Linearity | | To assess the linearity of the assay, samples were spiked with high concentrations of human IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | | Sample | Serum(n=4) | | | 1:1 | Average % | 94 | | | Range % | 90-98 | | | 1:2 | Average % | 98 | | | Range % | 94-103 | | | 1:4 | Average % | 97 | | | Range % | 90-106 | | | 1:8 | Average % | 94 | | | Range % | 88-109 | |
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| Recovery | | The recovery of human IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
| Sample Type | Average % Recovery | Range | | | Serum (n=5) | 92 | 87-97 | | | | | | | | | | | | | | | | | |
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| Typical Data | | These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 4000 | 2.872 | 2.752 | 2.812 | 2.705 | | | 2000 | 2.279 | 2.179 | 2.229 | 2.122 | | | 1000 | 1.545 | 1.589 | 1.567 | 1.460 | | | 500 | 0.952 | 1.002 | 0.977 | 0.870 | | | 250 | 0.531 | 0.575 | 0.553 | 0.446 | | | 125 | 0.341 | 0.366 | 0.354 | 0.247 | | | 62.5 | 0.255 | 0.275 | 0.265 | 0.158 | | | 0 | 0.108 | 0.105 | 0.107 | | |
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| Materials provided | - A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human IL-35 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled IL-35 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
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| Materials not provided | - A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
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| ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs | ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
