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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-EL011657RA] Rat Interleukin-34(IL34) ELISA kit
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[CSB-EL011657RA] Rat Interleukin-34(IL34) ELISA kit

제품코드 : CSB-EL011657RA

제품정보 : Rat Interleukin-34(IL34)

  • Sample Types: serum, plasma, tissue homogenates
  • Detect Range: 25 pg/mL-1600 pg/mL
  • Sensitivity: 6.25 pg/mL

제품문의

  • 전화번호
    2292-8870

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Product Details

Description

This Rat IL34 ELISA Kit was designed for the quantitative measurement of Rat IL34 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

Target Nameinterleukin 34
Alternative NamesIl34Interleukin-34 ELISA Kit; IL-34 ELISA Kit
AbbreviationIL34
Uniprot No.Q4KM46
SpeciesRattus norvegicus (Rat)
Sample Typesserum, plasma, tissue homogenates
Detection Range25 pg/mL-1600 pg/mL
Sensitivity6.25 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL34 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %89
Range %84-93
1:2Average %107
Range %103-110
1:4Average %85
Range %81-89
1:8Average %89
Range %84-93
Recovery
The recovery of rat IL34 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)107103-110
EDTA plasma (n=4)8683-89
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
16002.1002.1682.1341.995
8001.6541.6041.6291.490
4001.1811.2041.1931.054
2000.7650.7780.7720.633
1000.5170.5020.5100.371
500.3460.3510.3490.210
250.2280.2310.2300.091
00.1400.1380.139　
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

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